The enzyme that frustrates your weight loss efforts

You've been attempting to eat smaller portions and cut down on some foods entirely, but you're still not losing as much weight as you'd like. Well, a new study says that the complex action of one enzyme may be at the core of the problem.

Why do you struggle to lose weight, even when you think you're doing everything right?

Why do our bodies sometimes appear to turn against us, even as we do our best to stay in shape?

While we may adhere to a better diet and stop indulging in unhealthful foods, some of us will find it difficult to lose the excess weight that troubles us.

The reason behind why our bodies store fatty tissue in the first place is quite straightforward and even intuitive, given the nature of human evolution, explains Dr. Alan Saltiel, from the University of California, San Diego School of Medicine in La Jolla.

We derive energy by burning fat tissue, but sometimes, our bodies deem it necessary to curtail how much fat we burn so that we have enough "fuel" in store for later, when we may have more urgent need of it.

"Human bodies are very efficient at storing energy by repressing energy expenditure to conserve it for later when you need it," Dr. Saltiel notes, adding, "This is nature's way of ensuring that you survive if a famine comes."

Some of the mechanisms at play in this "fuel" storage and energy consumption system are unclear, however — particularly those related to the accumulation of excess fat that leads to obesity. The question is, what pushes the "on/off" button of fat metabolism, and when?

Dr. Saltiel and his team recently directed their attention toward the enzyme TANK-binding kinase 1 (TBK1), which they identified as key when it comes to the body's process of "deciding" how much fat to burn and how much to keep in store, especially over a period of fasting.

"There are two important observations that we have linked to slowing metabolism in obesity and fasting," explains Dr. Saltiel.

"We've discovered two new feedback loops that are intertwined to self-regulate the system. Think of it like your home thermostat, which senses change in temperature to turn heat off and on."

Dr. Alan Saltiel

The researchers' findings were reported today in the journal Cell.

Vicious metabolic cycles

Dr. Saltiel and team worked on the mouse model — using both obese and normal-weight animals — in order to study the role of TBK1 in metabolic processes. They noticed that the enzyme was implicated in two distinct processes, leading to the same result each time.

The first process is kick-started by obesity-related chronic stress, and it leads to inflammation as it activates a pro-inflammatory signaling pathway called NFKB.

NFKB enhances the expression of genes that "dictate" the production of enzymes thought to play a role in both inflammation and the accumulation of body fat, including the gene that encodes TBK1.

TBK1 then disactivates another enzyme, AMPK, which is largely responsible for regulating how much fat we convert into raw energy. This means that, instead of being burned, fat is able to accumulate and lead to excess weight.

The TBK1 enzyme is also implicated in the mechanism that is triggered by fasting. In fasting, the body's energy levels go down. The enzyme AMPK perceives that, and to boost energy, it sends signals to fat cells to convert into energy.

"This feedback loop blocks energy expenditure both through inflammation and fasting," Dr. Saltiel explains. When the scientists noticed this mechanism, they looked for a way to modify it.However, when AMPK is activated, it also boosts the expression of the TBK1 gene, which, once again, leads to the TBK1 enzyme inhibiting the activity of AMPK. A vicious cycle thus ensues, preventing the body from burning the accumulated fat.

"Energy expenditure was restored when we deleted TBK1 from fat cells [in] mice," he continues. "But something else occurred that surprised us — there was an increase in inflammation."

How can we 'restore energy balance?'

A second process with TBK1 at its core leads to an equally vicious cycle. The team also noted that, even as the NFKB pathway triggers the production of TBK1, the enzyme ends up inhibiting the NFKB pathway.

TBK1 normally helps to reduce inflammation without extinguishing it, however. Instead, it keeps it at low levels — when TBK1 is inactivated, the inflammatory response is heightened without regulatory action of the enzyme.

When Dr. Saltiel and colleagues deleted the TBK1 gene in obese mice, this triggered weight loss as well as increased inflammation. To the contrary, when TBK1 was deleted in normal-weight mice, no metabolic change was observed, suggesting that cutting down on calories could also help to reduce inflammation.

"Inhibiting TBK1 has the potential to restore energy balance in states of obesity by enhancing the ability to burn some fat," explains Dr. Saltiel.

While he notes that "[t]his is probably not the only pathway accounting for energy expenditure in fasting or obesity," he adds, "[T]his information provides new insight into how we might develop drugs that inhibit TBK1 or other enzymes involved in metabolism."

Still, the researchers note that taking special drugs won't be enough for those who want to be fitter.

"I think you'll probably still have to do both: reduce energy intake through diet and increase energy expenditure by blocking this compensatory reduction in burning calories," stresses Dr. Saltiel.